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November 1, 1995Circulation Research

Expression of cGMP-Dependent Protein Kinase I and Phosphorylation of Its Substrate, Vasodilator-Stimulated Phosphoprotein, in Human Endothelial Cells of Different Origin

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Population

Freshly isolated and cultured human endothelial cells from aorta, iliac artery, iliac vein, foreskin…

Design

Preclinical

Authors

RDRichard DraijerUnilever Foods Innovation CentreAVArie B. VaandragerUtrecht UniversityCNChristine NolteBielefeld University

Discussion

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Implication

cGMP-PK mediates selective endothelial barrier effects; leaves open its therapeutic relevance in vascular leak pending in vivo and clinical studies.

Structured PICO

P
Population
Freshly isolated and cultured human endothelial cells (ECs) from aorta, iliac artery, iliac vein, foreskin microvessels, and umbilical vein
I
Intervention
Selective cGMP-PK activator 8-(4-chlorophenylthio)-cGMP (8-pCPT-cGMP)
O
Outcome
VASP phosphorylation, thrombin-stimulated permeability, and cytoplasmic Ca2+ levelssurrogate

The study demonstrates that cGMP-PK type I is present in various human endothelial cells (except umbilical vein ECs) and mediates the reduction of thrombin-induced endothelial permeability via VASP phosphorylation.

Cite This Study

Draijer et al. (1995) studied this question.

synapsesocial.com/papers/6a7fb58ca3d55be9ef9fe8abhttps://doi.org/10.1161/01.res.77.5.897

Topics

Dual antiplatelet therapy
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1cGMP and Nitric Oxide Modulate Thrombin-Induced Endothelial Permeability1995 · 199 citations
  2. 2cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets.1994 · 470 citations
  3. 3Cellular mechanism of natriuretic peptides-induced inhibition of endothelin-1 biosynthesis in rat endothelial cells.1993 · 81 citations
  4. 4Passage of low density lipoproteins through monolayers of human arterial endothelial cells. Effects of vasoactive substances in an in vitro model.1989 · 56 citations
  5. 5Role of cyclic adenosine monophosphate in the induction of endothelial barrier properties1989 · 253 citations