NADPH oxidase mediates angiotensin II-induced endothelin-1 expression in vascular adventitial fibroblasts

Adventitial fibroblasts isolated and cultured from mouse aorta, including wild-type, gp91(phox) deleted, and SOD1 overexpressing models.

Angiotensin II (100 nmol/L) with or without NADPH oxidase inhibitors (apocynin 100 micromol/L, diphenyleneiodonium 10 micromol/L), superoxide scavengers (SOD 350 units/mL, tempol 100 micromol/L, tiron 100 micromol/L), and ET-receptor antagonists (BQ123, BQ788 10 microM).

Control cells or cells treated with Angiotensin II alone.

PreproET-1 mRNA and ET-1 peptide levels, superoxide anion production, and Type I procollagen-alpha-I expression.surrogate