June 1, 2000Arteriosclerosis Thrombosis and Vascular Biology

Inhibition of Platelet Integrin α IIb β 3 by Peptides That Interfere With Protein Kinases and the β 3 Tail

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Population

Human platelets permeabilized with streptolysin O

Comparison

Peptides interfering with Src kinases, protein… vs Intact platelets, reversed peptides, negative…

Design

Preclinical

Authors

IHIngeborg HersUniversity of California, San Francisco JDJosé DonathUtrecht University PLP.E.M.H. LitjensUtrecht University

Discussion

Member takes

Implication

Identifies beta3 domains regulating integrin activation; leaves open targeted antithrombotic development pending in vivo studies.

Structured PICO

Population

Human platelets permeabilized with streptolysin O

Intervention

Peptides interfering with Src kinases (YIYGSFK), protein kinase C (RKRCLRRL), and peptides mimicking the carboxy-terminal part of the beta3 subunit (NNPLYKEA, EATSTFTN, TNITYRGT)

Comparator

Intact platelets, reversed peptides (KFSGFIF), negative control peptides (parts of protein C inhibitor and beta2-glycoprotein I), and membrane-proximal domain peptide (LLITIHDR)

Outcome

alpha-thrombin-induced ligand binding to integrin alpha(IIb)beta(3)surrogate

Src kinases and protein kinase C participate in inside-out signaling to integrin alpha(IIb)beta(3), with specific target domains in the beta3 subunit contributing to its regulation.

Cite This Study

Hers et al. (2000) studied this question.

synapsesocial.com/papers/6a821b3ff07048b4a7ac8d54 https://doi.org/10.1161/01.atv.20.6.1651

Topics

Dual antiplatelet therapy

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Inhibition of Platelet Integrin α IIb β 3 by Peptides That Interfere With Protein Kinases and the β 3 Tail

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Topics

Inhibition of Platelet Integrin α IIb β 3 by Peptides That Interfere With Protein Kinases and the β 3 Tail

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Topics

Also Consider