What question did this study set out to answer?

To establish the quantitative relationship between graded decreases in oxygen tension and contractile depression in cultured myocardial cells devoid of intact tissue diffusion barriers.

November 1, 1980AJP Heart and Circulatory Physiology

Effects of graded hypoxia on contraction of cultured chick embryo ventricular cells

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Population

Single cells within spontaneously contracting monolayers cultured from 10-day-old chick embryo ventricles

Comparison

Graded hypoxia with or without inhibition of… vs Control levels (normoxia)

Design

Preclinical

Follow-up

3- to 4-min period of hypoxia

Authors

WBWilliam H. BarryBibliothèque Nationale de France JPJordan S. PoberUniversity of Jordan JMJames D. MarshAbu Dhabi National Oil (United Arab Emirates)

Discussion

Member takes

Implication

Glycolysis supports hypoxic contractility in isolated chick cells; leaves open translation to intact mammalian myocardium.

Key Points

To establish the quantitative relationship between graded decreases in oxygen tension and contractile depression in cultured myocardial cells devoid of intact tissue diffusion barriers.
Assessed contractility in spontaneously contracting monolayers of single ventricular cells isolated from 10-day-old chick embryos under varied perfusate oxygen tensions (Po2).
Measured cell wall motion amplitude, velocity, and beat rate during hypoxia and subsequent reoxygenation, both with and without 0.1 mM iodoacetate to inhibit glycolysis.
Perfusate Po2 below 12 mmHg triggered reversible reductions in contraction amplitude and velocity over 3 to 4 minutes, reaching maximum depression at approximately 30% of control levels below 4 mmHg.
Reoxygenation restored normal amplitude and velocity within 15 seconds.
Glycolytic inhibition with 0.1 mM iodoacetate increased the critical threshold for functional impairment to approximately 16 mmHg, exacerbating contraction loss and significantly slowing heart cell beating rate.

Structured PICO

Population

Single cells within spontaneously contracting monolayers cultured from 10-day-old chick embryo ventricles

Intervention

Graded hypoxia (perfusate Po2 < 12 mmHg and < 4 mmHg) with or without inhibition of glycolysis using 0.1 mM iodoacetate

Comparator

Control levels (normoxia)

Outcome

Amplitude and velocity of cell wall motion (contractility)surrogate

High-energy phosphates derived from anaerobic glycolysis can support a reduced level of contractility during brief periods of hypoxia in cultured chick embryo ventricular cells.

Cite This Study

Barry et al. (1980) studied this question.

synapsesocial.com/papers/6a826b7cfe5ce613f33acfdd https://doi.org/10.1152/ajpheart.1980.239.5.h651

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Effects of graded hypoxia on contraction of cultured chick embryo ventricular cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effects of graded hypoxia on contraction of cultured chick embryo ventricular cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider