Commonly used buffering agents HEPES and MOPS block chloride channels in Drosophila neurons, which may confound electrophysiological experiments.
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May confound Drosophila electrophysiology with HEPES/MOPS; leaves open effects in mammalian channels.
Chloride channels of neurons of Drosophila are blocked when the cytoplasmic side of the membrane is exposed to the commonly used buffering agents 4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid (HEPES) and 4-morpholinepropanesulphonic acid (MOPS). In the presence of these compounds, chloride channels appear to function as a complex of multiple protochannels.
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Yamamoto et al. (1987) studied this question.
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