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March 1, 1989Journal of VirologyOpen Access

Genetic variation occurring on the genome of an in vitro insertion mutant of poliovirus type 1

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Population

In vitro insertion mutant of poliovirus type 1 (Sabin strain)

Comparison

Insertion of a 72-nucleotide sequence from… vs Parent virus (Sabin strain)

Design

Preclinical

Authors

SKShusuke KugeTohoku Medical and Pharmaceutical UniversityNKNoriyuki KawamuraKishokai Medical CorporationANAkio NomotoTokyo Medical and Dental University

Discussion

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Implication

AUG in poliovirus 5' NCR may attenuate replication; leaves open vaccine applications pending human studies.

Structured PICO

P
Population
In vitro insertion mutant of poliovirus type 1 (Sabin strain)
I
Intervention
Insertion of a 72-nucleotide sequence from plasmid pUC18 at nucleotide position 702 of the 5' noncoding sequence
C
Comparator
Parent virus (Sabin strain)
O
Outcome
Plaque phenotype and nucleotide sequence of revertant genomes

An AUG sequence in the 5' noncoding region of the poliovirus type 1 genome is deleterious for efficient viral replication.

Cite This Study

Kuge et al. (1989) studied this question.

synapsesocial.com/papers/6a828cb292c19701ea21dc25https://doi.org/10.1128/jvi.63.3.1069-1075.1989
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