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January 1, 1988Proceedings of the National Academy of SciencesOpen Access

Construction of a "mutagenesis cartridge" for poliovirus genome-linked viral protein: isolation and characterization of viable and nonviable mutants.

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Population

Poliovirus type 1 (Mahoney) genome infectious cDNA clone

Comparison

Construction of a synthetic "mutagenesis… vs Wild-type poliovirus

Design

Preclinical

Authors

RKRichard KühnBavarian Academy of Sciences and HumanitiesHTH TadaTohoku UniversityMYM F Ypma-WongUniversity of California, Irvine

Discussion

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Implication

Tyrosine-RNA linkage is essential for poliovirus viability; hypothesis-generating for attenuation strategies but leaves open translation to vaccines or therapies.

Structured PICO

P
Population
Poliovirus type 1 (Mahoney) genome infectious cDNA clone
I
Intervention
Construction of a synthetic "mutagenesis cartridge" and insertion of mutations (methionine codons, alteration of tyrosine residue)
C
Comparator
Wild-type poliovirus
O
Outcome
Viral viability and growth characteristicssurrogate

The position of the tyrosine residue in the poliovirus genome-linked viral protein is essential for viral viability and function.

Cite This Study

Kühn et al. (1988) studied this question.

synapsesocial.com/papers/6a86c6b3cbc6304817beee96https://doi.org/10.1073/pnas.85.2.519
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Site-directed mutagenesis of proteinase 3C results in a poliovirus deficient in synthesis of viral RNA polymerase1987 · 58 citations
  2. 2Genome-linked protein VPg of poliovirus is present as free VPg and VPg-pUpU in poliovirus-infected cells.1983 · 93 citations
  3. 3Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.1983 · 157 citations
  4. 4Initiation of poliovirus plus-strand RNA synthesis in a membrane complex of infected HeLa cells1986 · 161 citations
  5. 5Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.1986 · 343 citations