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June 1, 1990Journal of VirologyOpen Access

Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg

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Population

HeLa cells transfected with nonviable poliovirus RNA mutated in the genome-linked protein VPg-coding region

Design

Preclinical

Authors

QRQuentin ReuerUniversity of Alaska AnchorageRKRichard KühnBavarian Academy of Sciences and HumanitiesEWEckard WimmerCalifornia Institute of Technology

Discussion

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Implication

Linkage-competent VPg is required for poliovirus RNA synthesis; extends replication models but leaves open in vivo and therapeutic relevance.

Structured PICO

P
Population
HeLa cells transfected with nonviable poliovirus RNA mutated in the genome-linked protein VPg-coding region
I
Intervention
Mutations in the VPg-coding region (lethal and nonlethal, including chimeric mutants)
O
Outcome
Viral RNA synthesis and replication

A linkage-competent VPg is necessary for poliovirus RNA synthesis, but other steps in replication can be interrupted by lethal mutations in VPg.

Cite This Study

Reuer et al. (1990) studied this question.

synapsesocial.com/papers/6a7c48287eebcb99d4a1613ahttps://doi.org/10.1128/jvi.64.6.2967-2975.1990
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Initiation of poliovirus plus-strand RNA synthesis in a membrane complex of infected HeLa cells1986 · 161 citations
  2. 2Purification and properties of a HeLa cell enzyme able to remove the 5'-terminal protein from poliovirus RNA.1980 · 57 citations
  3. 3Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosine1978 · 244 citations
  4. 4Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.1980 · 8,376 citations
  5. 5Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.1977 · 348 citations