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August 1, 1992Journal of VirologyOpen Access

A poliovirus replicon containing the chloramphenicol acetyltransferase gene can be used to study the replication and encapsidation of poliovirus RNA

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Population

Cells transfected with poliovirus replicon FLC/REP

Design

Preclinical

Authors

NPN PercyMi Corporation (United States)WBWilliam BarclayImperial Valley CollegeMSMichael L. SullivanAgricultural Research Service

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Implication

Enables efficient poliovirus replication studies in animal models; leaves open precise encapsidation signal mapping and in vivo translation.

Structured PICO

P
Population
Cells transfected with poliovirus replicon FLC/REP
I
Intervention
Transfection with FLC/REP replicon containing the chloramphenicol acetyltransferase (CAT) gene and mutations in the 5' noncoding region
O
Outcome
Replication efficiency, CAT enzyme activity, and encapsidation into poliovirus capsidssurrogate

A novel poliovirus replicon containing a CAT reporter gene enables efficient study of poliovirus RNA replication and encapsidation.

Cite This Study

Percy et al. (1992) studied this question.

synapsesocial.com/papers/6a8293b59192075d3cd4a661https://doi.org/10.1128/jvi.66.8.5040-5046.1992
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Also Consider

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  1. 1Expression of human immunodeficiency virus type 1 (HIV-1) gag, pol, and env proteins from chimeric HIV-1-poliovirus minireplicons1991 · 61 citations
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  3. 3In vitro construction of poliovirus defective interfering particles1989 · 88 citations
  4. 4The deletion of 41 proximal nucleotides reverts a poliovirus mutant containing a temperature-sensitive lesion in the 5' noncoding region of genomic RNA1989 · 68 citations
  5. 5Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine1989 · 235 citations