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July 1, 2000Microbiology

Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences

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Population

BHK cells electroporated with RNA transcripts of foot-and-mouth disease virus (FMDV) or derivatives

Comparison

Deletion or replacement of the leader and capsid… vs Full-length/wild-type FMDV RNA transcripts

Design

Preclinical

Authors

GMGerald M. McInerneyKarolinska InstitutetAKAndrew M. Q. KingThe Pirbright InstituteNRNatalie Ross-SmithThe Pirbright Institute

Discussion

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Implication

Lb/P1-deleted FMDV replicons replicate in animal models; leaves open optimized packaging for research vectors or applications.

Structured PICO

P
Population
BHK cells electroporated with RNA transcripts of foot-and-mouth disease virus (FMDV) or derivatives
I
Intervention
Deletion or replacement of the leader (Lab and Lb) and capsid protein coding sequences with sequences encoding chloramphenicol acetyltransferase (CAT)
C
Comparator
Full-length/wild-type FMDV RNA transcripts (implied)
O
Outcome
Expression of CAT and FMDV 3C protease, and ability of the RNA to replicatesurrogate

The study demonstrates that no cis-acting replication element is present within the Lb and P1 coding regions of the FMDV genome, and that TRANS-encapsidation of these replicons is very inefficient.

Cite This Study

McInerney et al. (2000) studied this question.

synapsesocial.com/papers/6a94493ecd4df3c00e00a3e5https://doi.org/10.1099/0022-1317-81-7-1699
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The foot-and-mouth disease virus leader proteinase gene is not required for viral replication1995 · 136 citations
  2. 2Role of 3'-end sequences in infectivity of poliovirus transcripts made in vitro1989 · 131 citations
  3. 3Defective Interfering Particles of Poliovirus I. Isolation and Physical Properties1971 · 153 citations
  4. 4Encapsidation studies of poliovirus subgenomic replicons.1998 · 49 citations
  5. 5Identification of a cis -Acting Replication Element within the Poliovirus Coding Region2000 · 250 citations