Population
Escherichia coli cells using the inducible system that utilizes T7 RNA polymerase
Design
Preclinical
Authors
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Supports scalable 2Apro production for in vitro studies; leaves open any clinical translation in viral disease.
Poliovirus protease 2Apro can be expressed at high levels in E. coli without toxicity and purified to 95% while retaining trans cleavage activity.
Martínez‐Abarca et al. (1993) studied this question.
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