What question did this study set out to answer?

To determine whether chick retina tissues synthesize platelet-activating factor (PAF) in response to neurotransmitters or calcium ionophores and to characterize the underlying enzymatic pathways.

December 1, 1986Journal of Biological ChemistryOpen Access

Production of platelet-activating factor by chick retina.

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Authors

FBFederico BussolinoCandiolo Cancer Institute FGFulvia GremoUniversity of Cagliari CTCiro TettaUniversity of Pisa

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Implication

In vitro experiment demonstrates neurotransmitter-stimulated synthesis of platelet-activating factor in chick retina, suggesting distinct pathway activation mechanisms.

Key Points

To determine whether chick retina tissues synthesize platelet-activating factor (PAF) in response to neurotransmitters or calcium ionophores and to characterize the underlying enzymatic pathways.
Chick retinal tissues and control cells (leukocytes, erythrocytes, fibroblasts) were stimulated with specific neurotransmitters or calcium ionophore A23187.
Extractable PAF was quantified and identified using chemical degradation, lipase treatments, washed rabbit platelet bioassays, thin-layer chromatography, and high-pressure liquid chromatography.
Enzymatic activity assays measured dithiothreitol-insensitive cholinephosphotransferase and acetyltransferase responses following agonist stimulation.
Acetylcholine, dopamine, and ionophore A23187 triggered cell-associated PAF production in a time- and concentration-dependent manner, whereas GABA, L-glycine, L-glutamate, epinephrine, and histamine did not.
Produced PAF remained tissue-associated rather than released into supernatants, with detection increasing markedly after tissue sonication.
Acetylcholine and dopamine drove PAF synthesis by enhancing dithiothreitol-insensitive cholinephosphotransferase activity without altering acetyltransferase, whereas A23187 selectively activated acetyltransferase.

Cite This Study

Bussolino et al. (1986) studied this question.

synapsesocial.com/papers/6a82bf4177f6571b411e0dc1 https://doi.org/10.1016/s0021-9258(18)66594-9

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Implication

In vitro experiment demonstrates neurotransmitter-stimulated synthesis of platelet-activating factor in chick retina, suggesting distinct pathway activation mechanisms.

Production of platelet-activating factor by chick retina.

Discussion

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Implication

Key Points

Cite This Study

Production of platelet-activating factor by chick retina.

Discussion

Member takes

Implication

Key Points

Cite This Study

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