What question did this study set out to answer?

To assess how the inhibition of nitric oxide synthesis by L-NAME influences blood pressure and tissue-specific uptake of LDL and fibrinogen in normotensive and hypertensive rats.

April 1, 1995British Journal of PharmacologyOpen Access

Effect of inhibition of nitric oxide synthesis on the uptake of LDL and fibrinogen by arterial walls and other organs of the rat

View Full Paper

Ask AI

Bookmark

Population

Conscious, unrestrained, cannulated normotensive and spontaneously hypertensive (SHR) Wistar rats

Comparison

3 mg ml-1 NG-nitro-L-arginine methyl ester… vs Control normotensive rats and baseline state for…

Design

Preclinical

Follow-up

6 days

Authors

LCL E Cardona-SanclementeUniversité Paris-Sud GBG. V. R. BornUniversity of London

Discussion

Member takes

Implication

Hypertension status may modulate NO inhibition effects on aortic uptake; hypothesis-generating in animal models only.

Key Points

To assess how the inhibition of nitric oxide synthesis by L-NAME influences blood pressure and tissue-specific uptake of LDL and fibrinogen in normotensive and hypertensive rats.
Administered 3 mg/ml L-NAME via drinking water for 6 days to conscious, cannulated normotensive and spontaneously hypertensive Wistar rats.
Measured blood pressure and quantified [125I]TC-LDL and [131I]TC-fibrinogen uptake across aortic wall, heart, skeletal muscle, lung, liver, kidney, and adrenal tissues during the final 24 hours.
In normotensive rats, L-NAME increased systolic blood pressure progressively to approximately 170 mmHg and significantly elevated uptake of both LDL and fibrinogen in aortic walls and heart, but not in muscle, lung, liver, kidney, or adrenal glands.
In spontaneously hypertensive rats, baseline blood pressure was approximately 170 mmHg and did not increase significantly with L-NAME; LDL and fibrinogen uptake rose significantly only in the heart, with no increase in the aorta or other organs.

Structured PICO

Population

Conscious, unrestrained, cannulated normotensive and spontaneously hypertensive (SHR) Wistar rats

Intervention

3 mg ml-1 NG-nitro-L-arginine methyl ester (L-NAME) administered in drinking water for 6 days

Comparator

Control normotensive rats and baseline state for SHR

Outcome

Uptake of low density lipoprotein (LDL) and fibrinogen in aortic walls, heart, skeletal muscle, lung, liver, kidney, and adrenal, and blood pressuresurrogate

Inhibition of nitric oxide synthesis increases blood pressure and the uptake of atherogenic plasma proteins in the aorta and heart of normotensive rats, but not in the aorta of spontaneously hypertensive rats.

Cite This Study

Cardona-Sanclemente et al. (1995) studied this question.

synapsesocial.com/papers/6a82c4431d8eddfbf50f732b https://doi.org/10.1111/j.1476-5381.1995.tb13375.x

View Full Paper

Ask AI

Bookmark

Effect of inhibition of nitric oxide synthesis on the uptake of LDL and fibrinogen by arterial walls and other organs of the rat

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effect of inhibition of nitric oxide synthesis on the uptake of LDL and fibrinogen by arterial walls and other organs of the rat

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider