May 6, 2006AJP Heart and Circulatory Physiology
Angiopoietin-1-induced angiogenesis is modulated by endothelial NADPH oxidase
Population
Porcine coronary artery endothelial cells, mouse heart microvascular endothelial cells from wild-type and…
Comparison
Angiopoietin-1 250 ng/ml, with or without NADPH… vs Untreated cells/tissues, or wild-type…
Design
Preclinical
Authors
Discussion
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Implication
Supports NADPH oxidase mediation of Ang-1 angiogenesis in preclinical models; leaves open therapeutic targeting in human vascular disease.
Structured PICO
P
Population
Porcine coronary artery endothelial cells (PCAECs), mouse heart microvascular endothelial cells from wild-type (WT) and p47(phox-/-) mice, and aortic rings from WT and p47(phox-/-) mice.
I
Intervention
Angiopoietin-1 (Ang-1) 250 ng/ml, with or without NADPH oxidase inhibitors (DPI 10 microM, apocynin 200 microM) or Tie-2 receptor inhibition.
C
Comparator
Untreated cells/tissues, or wild-type (WT) cells/tissues compared to p47(phox-/-) cells/tissues.
O
Outcome
Intracellular ROS production, Akt and p44/42 MAPK phosphorylation, endothelial cell migration, and vessel sprouting.surrogate
Endothelial NADPH oxidase-derived reactive oxygen species are critical mediators of Angiopoietin-1-induced angiogenesis.
Cite This Study
Chen et al. (2006) studied this question.