February 8, 2011PLoS ONEOpen Access

A Local Proinflammatory Signalling Loop Facilitates Adverse Age-Associated Arterial Remodeling

Q: What is the clinical evidence from this study?

Study design: Other. Population: Age-associated arterial remodeling (n=40). Intervention: MCP-1, TGF-b1, and MMP-2 modulation vs. Untreated cells / Young vs Old. Primary outcome: Vascular smooth muscle cell (VSMC) invasion and collagen production (p=<0.05).

View Full Paper

Ask AI

Bookmark

Key result

An autocatalytic signaling loop involving MCP-1, MMP-2, and TGF-β1 enhances collagen production and invasiveness of vascular smooth muscle cells, contributing to adverse age-associated arterial remodeling.

Why the study?

Does suppression of the MCP-1/MMP-2/TGF-β1 signaling loop prevent age-associated adverse arterial remodeling in rat VSMCs?

Population

Male Fisher 344 crossbred Brown Norway rats, 8-month-old and 30-month-old, and vascular smooth muscle cells…

Comparison

In vitro treatments including MCP-1, TGF-β1… vs Untreated VSMCs or scramble siRNA controls.

Design

Preclinical

Authors

MWMingyi Wang GSGaia Spinetti RMRobert E. Monticone

Discussion

Member takes

Overview

Suggests a novel target against vascular aging; leaves open therapeutic efficacy pending prospective validation.

Structured PICO

Does suppression of the MCP-1/MMP-2/TGF-β1 signaling loop prevent age-associated adverse arterial remodeling in rat VSMCs?

Population

40 male Fisher 344 crossbred Brown Norway rats aged 8 and 30 months used to study the molecular mechanisms of age-associated arterial remodeling.

Intervention

In vitro treatments including MCP-1 (1-100 ng/ml), TGF-β1 (1-100 ng/ml), si-MCP-1 (25-50 nM), CCR2 antagonist vCCI (150 ng/ml), and MMP-2 inhibitor GM 6001 (15 μM) or neutralizing antibody.

Comparator

Untreated VSMCs or scramble siRNA controls.

Outcome

Activation of the MCP-1/MMP-2/TGF-β1 signaling loop, collagen production, and VSMC invasiveness.surrogate

Main Result

p-value: p=<0.05

A local proinflammatory signaling loop involving MCP-1, MMP-2, and TGF-β1 facilitates adverse age-associated arterial remodeling, providing a potential therapeutic target to retard vascular aging.

Limitations

In vitro and animal model findings may not fully translate to human arterial aging
Complex signaling networks and other suppressive signaling pathways were not fully explored

Cite This Study

Wang et al. (2011) studied Age-associated arterial remodeling (n=40). MCP-1, TGF-b1, and MMP-2 modulation vs. Untreated cells / Young vs Old was evaluated on Vascular smooth muscle cell (VSMC) invasion and collagen production (p=<0.05). An autocatalytic signaling loop involving MCP-1, MMP-2, and TGF-β1 enhances collagen production and invasiveness of vascular smooth muscle cells, contributing to adverse age-associated arterial remodeling.

synapsesocial.com/papers/6a82d03072d82bb027239db8 https://doi.org/10.1371/journal.pone.0016653

View Full Paper

Ask AI

Bookmark

February 8, 2011PLoS ONEOpen Access

A Local Proinflammatory Signalling Loop Facilitates Adverse Age-Associated Arterial Remodeling

View Full Paper

Ask AI

Bookmark

Key result

Why the study?

Does suppression of the MCP-1/MMP-2/TGF-β1 signaling loop prevent age-associated adverse arterial remodeling in rat VSMCs?

Population

Male Fisher 344 crossbred Brown Norway rats, 8-month-old and 30-month-old, and vascular smooth muscle cells…

Comparison

In vitro treatments including MCP-1, TGF-β1… vs Untreated VSMCs or scramble siRNA controls.

Design

Preclinical

Authors

MWMingyi Wang GSGaia Spinetti RMRobert E. Monticone

Discussion

Member takes

Overview

Suggests a novel target against vascular aging; leaves open therapeutic efficacy pending prospective validation.

Structured PICO

Does suppression of the MCP-1/MMP-2/TGF-β1 signaling loop prevent age-associated adverse arterial remodeling in rat VSMCs?

Population

40 male Fisher 344 crossbred Brown Norway rats aged 8 and 30 months used to study the molecular mechanisms of age-associated arterial remodeling.

Intervention

In vitro treatments including MCP-1 (1-100 ng/ml), TGF-β1 (1-100 ng/ml), si-MCP-1 (25-50 nM), CCR2 antagonist vCCI (150 ng/ml), and MMP-2 inhibitor GM 6001 (15 μM) or neutralizing antibody.

Comparator

Untreated VSMCs or scramble siRNA controls.

Outcome

Activation of the MCP-1/MMP-2/TGF-β1 signaling loop, collagen production, and VSMC invasiveness.surrogate

Main Result

p-value: p=<0.05

A local proinflammatory signaling loop involving MCP-1, MMP-2, and TGF-β1 facilitates adverse age-associated arterial remodeling, providing a potential therapeutic target to retard vascular aging.

Limitations

In vitro and animal model findings may not fully translate to human arterial aging
Complex signaling networks and other suppressive signaling pathways were not fully explored

Cite This Study

synapsesocial.com/papers/6a82d03072d82bb027239db8 https://doi.org/10.1371/journal.pone.0016653

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark