Key result
Bath application of angiotensin II significantly increased sympathetic nerve activity to 124% of control in a novel in vitro newborn rat brainstem-spinal cord preparation.
This novel in vitro brainstem-spinal cord preparation preserves functional connections between the medullary cardiovascular center and peripheral sympathetic outputs, enabling detailed study of central sympathetic control.
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This preclinical model has no immediate clinical application; extends research capabilities by providing a novel in vitro platform to.
Newborn rat brainstem-spinal cord preparations are useful for in vitro analysis of various brainstem functions including respiratory activity. When studying the central control of sympathetic nerve activity (SNA), it is important to record peripheral outputs of the SNA. We developed an in vitro preparation in which neuronal connections between the cardiovascular center in the medulla and SNA peripheral outputs are preserved. Zero- to 1-day-old rats were deeply anesthetized with isoflurane, and the brainstem and spinal cord were isolated with a partial right thoracic cage to record sympathetic nerve discharge from the right thoracic sympathetic nerve trunk (T9-T11). SNA in this preparation was strongly modulated by inspiratory activity. Single-shot electrical stimulation of the ipsilateral rostral ventrolateral medulla (RVLM) induced a transient increase of SNA. Bath application of angiotensin II induced an increase of SNA, and local ipsilateral microinjection of angiotensin II to the RVLM induced a transient increase of SNA. This preparation allows analysis of the central control of the SNA in vitro.
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Oyama et al. (2017) studied Healthy newborn rats (in vitro model development). Angiotensin II vs. Control (baseline) was evaluated on Maximum amplitude of integrated sympathetic nerve activity (SNA) as a percentage of control (124 ± 11.4% of control, p=<0.05). Bath application of angiotensin II significantly increased sympathetic nerve activity to 124% of control in a novel in vitro newborn rat brainstem-spinal cord preparation.
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