November 1, 1998ViewOpen Access

A Ras-Dependent Pathway Regulates RNA Polymerase II Phosphorylation in Cardiac Myocytes: Implications for Cardiac Hypertrophy

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Structured PICO

Population

Neonatal cardiac myocytes cultured from 1- to 2-day-old Sprague-Dawley rats, and 20- to 30-g, 12-week-old adult FVB mice

Intervention

Recombinant adenoviruses harboring a dominant negative Ras (17N Ras) allele or the N-terminal domain of GAP (nGAP); phenylephrine stimulation; transverse aortic constriction (aortic banding)

Comparator

Control adenoviruses (Ad.SV, Ad.CMV); vehicle; sham operation

Outcome

RNA polymerase II (pol II) phosphorylation, Cdk7 expression, and global RNA and protein synthesis (measured by [3H]uridine and [3H]phenylalanine incorporation)surrogate

A Ras-dependent signaling pathway regulates global RNA and protein synthesis in cardiac myocytes via phosphorylation of RNA polymerase II, providing a molecular mechanism for the development of cardiac hypertrophy.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Ras inhibition does not yet inform clinical hypertrophy management; leaves open pathway targeting for human validation.

Cite This Study

Abdellatif et al. (1998) studied this question.

synapsesocial.com/papers/6a82d876c40fc255ce1346af https://doi.org/10.1128/mcb.18.11.6729

View Full Paper

Ask AI

Bookmark

A Ras-Dependent Pathway Regulates RNA Polymerase II Phosphorylation in Cardiac Myocytes: Implications for Cardiac Hypertrophy

Structured PICO

Discussion

Implication

Cite This Study

A Ras-Dependent Pathway Regulates RNA Polymerase II Phosphorylation in Cardiac Myocytes: Implications for Cardiac Hypertrophy

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider