Determination of ascorbic acid by flow injection with chemiluminescence detection

Flow injection was used in combination with chemiluminescence detection to determine 30 amol of ascorbic acid based on a luminol–Fe3+–hydrogen peroxide system. The method was applied to the determination of ascorbic acid in pure form, pharmaceutical preparations and fruit juices. The method is sensitive, rapid (sampling rate 2–56 samples h–1) and tolerates the presence of common ingredients usually found in fruit juices and drug formulations.