Alkaline buffers release EDRF from bovine cultured aortic endothelial cells

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Population

Bovine cultured aortic endothelial cells

Comparison

Alkaline buffers increasing pH to 8.6-9.5 vs Standard Krebs solution or other agonists

Design

Preclinical

Authors

Discussion

Alkalinisation may modulate endothelial EDRF release in vitro; hypothesis-generating for vascular effects of pH in vivo.

Population

Bovine cultured aortic endothelial cells

Intervention

Alkaline buffers (e.g., Sodium carbonate, L-Arginine free base, D-arginine free base) increasing pH to 8.6-9.5

Comparator

Standard Krebs solution (pH 7.5-8.0) or other agonists (bradykinin, ADP)

Outcome

Release of endothelium-derived relaxing factor (EDRF) and prostacyclin (PGI2)surrogate

Alkalinisation of the extracellular environment stimulates the release of EDRF from endothelial cells through a calcium-dependent mechanism.

Mitchell et al. (1991) studied this question.

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