October 1, 2002Nucleic Acids ResearchOpen Access

The bulge region of HIV-1 TAR RNA binds metal ions in solution

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Population

Chemically synthesised 29mer oligoribonucleotides of the HIV-1 TAR sequence labelled with 5-fluorouridine…

Design

Preclinical

Authors

MOMikołaj OlejniczakAdam Mickiewicz University in Poznań

Discussion

Member takes

Implication

No immediate implications for HIV care; leaves open whether weak divalent cation binding modulates TAR function in cellular or in vivo models.

Structured PICO

Population

Chemically synthesised 29mer oligoribonucleotides of the HIV-1 TAR sequence labelled with 5-fluorouridine (FU) and 20 NMR conformers (PDB code 1ANR)

Intervention

Titration with Mg2+, Ca2+, Co(NH3)6(3+) ions and argininamide

Outcome

Metal ion binding affinity (Kd) and cleavage patterns at the bulge region

The study demonstrates that divalent metal ions like Mg2+ and Ca2+ bind specifically, albeit weakly, to the bulge region of HIV-1 TAR RNA in solution.

Cite This Study

Mikołaj Olejniczak (2002) studied this question.

synapsesocial.com/papers/6a8386adafb287de3d9608eb https://doi.org/10.1093/nar/gkf541

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Implication

No immediate implications for HIV care; leaves open whether weak divalent cation binding modulates TAR function in cellular or in vivo models.

The bulge region of HIV-1 TAR RNA binds metal ions in solution

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The bulge region of HIV-1 TAR RNA binds metal ions in solution

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider