What question did this study set out to answer?

To investigate whether the nitric oxide/cGMP signaling pathway mediates the biphasic regulation of Na+-K+-ATPase activity by angiotensin II in rat proximal tubules.

March 1, 2001AJP Renal Physiology

NO/cGMP signaling modulates regulation of Na+-K+-ATPase activity by angiotensin II in rat proximal tubules

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Population

Freshly isolated rat proximal tubules

Comparison

Angiotensin II at varying concentrations, with… vs Control (baseline activity without ANG II)

Design

Preclinical

Authors

CZChaojie ZhangUniversity of Illinois Urbana-Champaign PMPhilip R. MayeuxUniversity of Arkansas for Medical Sciences

Discussion

Member takes

Implication

Preclinical data only; leaves open whether NO/cGMP feedback modulates ANG II effects on human tubular sodium transport.

Key Points

To investigate whether the nitric oxide/cGMP signaling pathway mediates the biphasic regulation of Na+-K+-ATPase activity by angiotensin II in rat proximal tubules.
Measured Na+-K+-ATPase activity in freshly isolated rat proximal tubules exposed to angiotensin II across concentrations ranging from 10^-13 M to 10^-7 M.
Treated tubule suspensions with L-arginine, the nitric oxide synthase inhibitor L-NMMA, the soluble guanylyl cyclase inhibitor ODQ, or the AT1 receptor antagonist SK&F 108566, alongside measuring intracellular cGMP.
Angiotensin II induced biphasic regulation of Na+-K+-ATPase, peaking at 10^-11 M (1.43 ± 0.08-fold above control) and declining to baseline at 10^-7 M (0.96 ± 0.1-fold), with low-dose stimulation blocked by the AT1 antagonist SK&F 108566.
Inhibition of nitric oxide synthase with L-NMMA or soluble guanylyl cyclase with ODQ restored stimulation at 10^-7 M to 1.40 ± 0.1-fold and 1.30 ± 0.1-fold, respectively, while 10^-7 M angiotensin II increased intracellular cGMP 1.58 ± 0.28-fold.

Structured PICO

Population

Freshly isolated rat proximal tubules

Intervention

Angiotensin II (ANG II) at varying concentrations (10^-13 to 10^-7 M), with or without L-arginine, L-NMMA, 1H-[1,2,4]oxadiazole-[4,3-a]quinoxalin-1-one, or SK&F 108566

Comparator

Control (baseline activity without ANG II)

Outcome

Na+-K+-ATPase activitysurrogate

The NO/cGMP signaling pathway serves as a negative feedback mechanism in the regulation of Na+-K+-ATPase activity by angiotensin II in rat proximal tubules.

Cite This Study

Zhang et al. (2001) studied this question.

synapsesocial.com/papers/6a83b70bf72f0ca72062c9e1 https://doi.org/10.1152/ajprenal.2001.280.3.f474

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NO/cGMP signaling modulates regulation of Na+-K+-ATPase activity by angiotensin II in rat proximal tubules

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

NO/cGMP signaling modulates regulation of Na+-K+-ATPase activity by angiotensin II in rat proximal tubules

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider