July 12, 2012Bangladesh Journal of Veterinary MedicineOpen Access

Standardization of Multiplex Reverse Transcription-Polymerase Chain Reaction and Typing of Foot-and-Mouth Disease Virus Prevalent in Bangladesh

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Structured PICO

Population

82 field samples (vesicular fluid, tongue epithelium and tissue from inter-digital space) from cattle in Bangladesh from the years 2007 and 2008

Intervention

Multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay

Outcome

Detection and differentiation of foot-and-mouth disease virus (FMDV) serotypes

A standardized multiplex RT-PCR assay successfully detected and differentiated FMDV serotypes in field samples from cattle, which could aid in strategic vaccination programs.

Discussion

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Implication

RT-PCR positivity rates support sample prioritization for FMDV surveillance; leaves open translation to vaccination programs pending validation.

Cite This Study

Zinnah et al. (2012) studied this question.

synapsesocial.com/papers/6a83fec7e860903c4d4d88db https://doi.org/10.3329/bjvm.v8i2.11199

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Implication

RT-PCR positivity rates support sample prioritization for FMDV surveillance; leaves open translation to vaccination programs pending validation.

Standardization of Multiplex Reverse Transcription-Polymerase Chain Reaction and Typing of Foot-and-Mouth Disease Virus Prevalent in Bangladesh

Structured PICO

Discussion

Implication

Cite This Study

Standardization of Multiplex Reverse Transcription-Polymerase Chain Reaction and Typing of Foot-and-Mouth Disease Virus Prevalent in Bangladesh

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider