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February 1, 2001Journal of VirologyOpen Access

Characterization of Recombinant Hepatitis A Virus Genomes Containing Exogenous Sequences at the 2A/2B Junction

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Population

Fetal rhesus kidney cells and African green monkey kidney cells used for rescue and assay of infectious…

Comparison

Recombinant HAV genomes containing exogenous… vs Parent recombinant HAV clone (p18f)

Design

Preclinical

Authors

MBMichael R. BeardThe University of AdelaideLCLisette CohenSLStanley M. LemonUniversity of North Carolina at Chapel Hill

Discussion

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Implication

May enable HAV-based expression vectors; leaves open in vivo stability and therapeutic translation.

Structured PICO

P
Population
Fetal rhesus kidney (FRhK-4) cells and African green monkey kidney cells (BS-C-1) used for rescue and assay of infectious Hepatitis A virus (HAV)
I
Intervention
Recombinant HAV genomes containing exogenous sequences (IRES or protein-coding sequences like Zeo) inserted at the 2A/2B junction
C
Comparator
Parent recombinant HAV clone (p18f)
O
Outcome
Rescue of infectious virus and replication efficiencysurrogate

The HAV polyprotein can tolerate insertions at the 2A/2B junction, demonstrating that the 2A polypeptide does not function in cis as a 2AB precursor and allowing for the development of novel HAV expression vectors.

Cite This Study

Beard et al. (2001) studied this question.

synapsesocial.com/papers/6a8441c87b4d6f59a47c00a6https://doi.org/10.1128/jvi.75.3.1414-1426.2001
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Morphogenesis of hepatitis A virus: isolation and characterization of subviral particles1990 · 91 citations
  2. 2Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence1991 · 494 citations
  3. 3Maturation of the Hepatitis A Virus Capsid Protein VP1 Is Not Dependent on Processing by the 3C pro Proteinase1999 · 47 citations
  4. 4Characterization of Replication-Competent Hepatitis A Virus Constructs Containing Insertions at the N Terminus of the Polyprotein1998 · 14 citations
  5. 5Synthesis and assembly of hepatitis A virus-specific proteins in BS-C-1 cells1993 · 40 citations