Improving Proteolytic Cleavage at the 3A/3B Site of the Hepatitis A Virus Polyprotein Impairs Processing and Particle Formation, and the Impairment Can Be Complemented in trans by 3AB and 3ABC

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Population

Recombinant vaccinia virus system expressing Hepatitis A virus polyprotein and in vitro transcripts of…

Comparison

Mutations altering the amino acid sequence at… vs Wild-type HAV polyprotein

Design

Preclinical

Authors

Discussion

Reducing 3A/3B cleavage impairs HAV assembly in models; leaves open antiviral targeting of polyprotein processing.

Population

Recombinant vaccinia virus system expressing Hepatitis A virus (HAV) polyprotein and in vitro transcripts of cleavage site mutants

Intervention

Mutations altering the amino acid sequence at the 3A/3B and 3B/3C cleavage sites to improve cleavage

Comparator

Wild-type HAV polyprotein

Outcome

HAV polyprotein processing, particle formation, and viral replicationsurrogate

Accumulation of uncleaved HAV 3AB and/or 3ABC is pivotal for both viral replication and efficient particle formation.

Kusov et al. (1999) studied this question.

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