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April 1, 1993Journal of General Virology

Cell-free translation and proteolytic processing of the hepatitis A virus polyprotein

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Population

In vitro model using rabbit reticulocyte lysates for expressing genomic regions of hepatitis A virus (HAV)

Design

Preclinical

Authors

DJDirk JürgensenUniversity of LübeckYKYuri KusovUniversity of LübeckMFM FäckeGerman Cancer Research Center

Discussion

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Implication

Should not yet inform HAV treatment; leaves open targeted 3C protease inhibitor development in humans.

Structured PICO

P
Population
In vitro model using rabbit reticulocyte lysates for expressing genomic regions of hepatitis A virus (HAV)
I
Intervention
In vitro transcription and translation of viral polyprotein segments (P1-P2, delta VP1-P2, P3 region, and mutant transcripts)
O
Outcome
Proteolytic processing and cleavage of the viral polyproteinsurrogate

The study demonstrates that hepatitis A virus proteinase activity is encoded within the 3C region and requires a cysteine residue at position 172, functioning as a cysteine-containing trypsin-like proteinase.

Limitations

  • Analysis of cleavage products was made difficult by the presence of polypeptides translated from internal start sites
  • Possible cleavage of P1 and P2 by 3C could not be resolved due to small amounts of full-length products translated
  • No intermolecular cleavage could be detected

Cite This Study

Jürgensen et al. (1993) studied this question.

synapsesocial.com/papers/6a891db06abbd4965f28b34fhttps://doi.org/10.1099/0022-1317-74-4-677
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Intermolecular cleavage of hepatitis A virus (HAV) precursor protein P1-P2 by recombinant HAV proteinase 3C1992 · 15 citations
  2. 2Poliovirus polypeptide precursors: expression in vitro and processing by exogenous 3C and 2A proteinases.1987 · 126 citations
  3. 3Processing determinants required for in vitro cleavage of the poliovirus P1 precursor to capsid proteins1987 · 74 citations
  4. 4Poliovirus Protein 3CD Is the Active Protease for Processing of the Precursor Protein P1 in vitro1988 · 188 citations
  5. 5Complete nucleotide sequence of wild-type hepatitis A virus: comparison with different strains of hepatitis A virus and other picornaviruses1987 · 385 citations