What is the clinical evidence from this study?

Study design: Other. Population: Classical swine fever virus (CSFV) infection in cultured cells. Intervention: END- CSFV infection vs. END+ CSFV infection or mock infection. Primary outcome: Cytopathic effect (CPE) and accumulation of NS3 protein.

January 1, 2004Journal of Veterinary Medical ScienceOpen Access

Cytopathogenicity of Classical Swine Fever Viruses that do not Show the Exaltation of Newcastle Disease Virus is Associated with Accumulation of NS3 in Serum-Free Cultured Cell Lines

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Key result

Cytopathic effect in serum-free cultured cell lines infected with END- classical swine fever viruses is closely associated with the accumulation of the NS3 protein.

Population

Porcine cell lines (FS-L3, CPK-NS, PK-15, SKp, STp) infected with Classical Swine Fever Viruses (CSFV)

Comparison

Infection with END CSFV strains vs Infection with END CSFV strains and…

Design

Preclinical

Follow-up

72 hours

Authors

HAHiroshi AokiYokohama National University YSYoshihiro SakodaHealth Sciences University of Hokkaido SNShigeyuki NakamuraMinistry of Agriculture, Forestry and Fisheries

Discussion

Member takes

Implication

NS3 accumulation associates with cytopathogenicity in END(-) CSFV cell lines; hypothesis-generating for swine fever mechanisms.

Structured PICO

Population

In vitro study evaluating the cytopathogenic phenotype of classical swine fever viruses in porcine cell lines.

Exposure

Infection with END(-) CSFV strains (ALD-END-, Ames-END-, GPE-)

Comparator

Infection with END(+) CSFV strains (ALD, Ames, WB82/E+) and mock-infected cells

Outcome

Cytopathic effect (CPE) and quantification of nonstructural proteins NS2-3 and NS3surrogate

Cytopathogenicity of END(-) classical swine fever viruses in serum-free cultured cells is associated with the accumulation of the NS3 protein.

Limitations

Further studies are needed to determine whether cytopathogenicity is due to NS3 expression levels or NS2-3 cleavage efficiency.
It remains unclear whether the detachment of PK-15 cells infected with END- virus relates to NS3 accumulation or another factor.

Cite This Study

Aoki et al. (2004) studied Classical swine fever virus (CSFV) infection in cultured cells. END- CSFV infection vs. END+ CSFV infection or mock infection was evaluated on Cytopathic effect (CPE) and accumulation of NS3 protein. Cytopathic effect in serum-free cultured cell lines infected with END- classical swine fever viruses is closely associated with the accumulation of the NS3 protein.

synapsesocial.com/papers/6a844f7bf6d812dcfa83dca2 https://doi.org/10.1292/jvms.66.161

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Cytopathogenicity of Classical Swine Fever Viruses that do not Show the Exaltation of Newcastle Disease Virus is Associated with Accumulation of NS3 in Serum-Free Cultured Cell Lines

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Cytopathogenicity of Classical Swine Fever Viruses that do not Show the Exaltation of Newcastle Disease Virus is Associated with Accumulation of NS3 in Serum-Free Cultured Cell Lines

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider