September 1, 1995AJP Heart and Circulatory Physiology

Increase of cardiac work is associated with decrease of mitochondrial NADH

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Population

Isolated rat hearts perfused according to Langendorff (n=6 per substrate group)

Comparison

Increase in cardiac work during perfusion with… vs Baseline cardiac work

Design

Preclinical

Authors

JAJ. F. AshrufUniversity of Groningen JCJ. M. C. C. CoremansThe Netherlands Cancer Institute HBHajo A. BruiningErasmus MC

Discussion

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Implication

Challenges NADH/NAD+ redox as primary respiratory driver in isolated hearts; leaves open translation to human cardiac metabolism.

Structured PICO

Population

Isolated rat hearts perfused according to Langendorff (n=6 per substrate group)

Intervention

Increase in cardiac work (heartbeat rate raised from 5 to 7 Hz, left ventricular pressure raised stepwise from 0 to +/- 90 mmHg) during perfusion with 5.5 mM glucose or 10 mM pyruvate

Comparator

Baseline cardiac work

Outcome

Mitochondrial NADH/NAD+ measured via epicardial autofluorescencesurrogate

In isolated rat hearts, increased cardiac work and oxygen consumption lead to a decrease in mitochondrial NADH, suggesting that a rise in NADH/NAD+ is not the primary stimulus for increased respiration.

Cite This Study

Ashruf et al. (1995) studied this question.

synapsesocial.com/papers/6a845d49ac510c864ff30dee https://doi.org/10.1152/ajpheart.1995.269.3.h856

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Increase of cardiac work is associated with decrease of mitochondrial NADH

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Increase of cardiac work is associated with decrease of mitochondrial NADH

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider