Interaction of avian reovirus with chicken lymphoblastoid cell lines

Four chicken lymphoblastoid cell lines were inoculated with avian reovirus strain S1133 and two local isolates, 965 and 615. Of the inoculated cell lines, TLT, a B-cell line, was productively infected with the three viruses as demonstrated by immunofluorescence assay (IFA) and radioimmunoprecipitation assay. A comparative growth curve analysis of the three avian reoviruses was done at 37 degrees and 41 degrees C. Isolate 965 replicated to a higher titre at both temperatures while the replication of S1133 and 615 was found to be inhibited at 41 degrees C. IFA revealed that among the transformed T lymphoblastoid cells used in this study, only MDCC-RP1 was permissive to virus infection with isolate 965, and at 41 degrees C, but not 37 degrees C.