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August 1, 1989Proceedings of the National Academy of SciencesOpen Access

20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition.

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Population

HeLa cells and sera from patients with systemic lupus erythematosus or mixed connective tissue disease

Design

Preclinical

Authors

MBM.A. BachInstitute of Molecular BiotechnologyGWG. WinkelmannUniversity of TübingenRLReinhard LührmannMax Planck Institute for Multidisciplinary Sciences

Discussion

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Implication

Points to protein-driven U5 snRNP activity; leaves open functional validation and relevance to human splicing disorders.

Structured PICO

P
Population
HeLa cells (S3) and sera from patients with systemic lupus erythematosus (SLE) or mixed connective tissue disease (MCTD)
I
Intervention
Purification of U5 snRNP from HeLa nuclear splicing extracts
O
Outcome
Protein composition and sedimentation constant of U5 snRNP

U5 snRNP contains multiple unique high molecular mass proteins, suggesting it may exert its splicing function mainly through its protein components.

Cite This Study

Bach et al. (1989) studied this question.

synapsesocial.com/papers/6a848915c0e58a00207c0012https://doi.org/10.1073/pnas.86.16.6038
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 15'-terminal caps of snRNAs are accessible for reaction with 2,2,7-trimethylguanosine-specific antibody in intact snRNPs.1983 · 67 citations
  2. 2Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.1979 · 53,820 citations
  3. 3Separation of multiple components of HeLa cell nuclear extracts required for pre-messenger RNA splicing.1987 · 56 citations
  4. 4Immunization of mice with purified U1 small nuclear ribonucleoprotein (RNP) induces a pattern of antibody specificities characteristic of the anti-Sm and anti-RNP autoimmune response of patients with lupus erythematosus, as measured by monoclonal antibodies.1986 · 85 citations