January 1, 1988Nucleic Acids ResearchOpen Access

Pre-mRNA splicing by complementation with purified human UI, U2, U4/U6 and U5 snRNPs

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Population

HeLa cell nuclear extracts active in splicing

Design

Preclinical

Authors

AKAdrian R. KrainerUniversity of Vienna

Discussion

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Implication

Indicates spliceosome activity requires accessory proteins beyond snRNPs; leaves open their identities and in vivo roles.

Structured PICO

Population

HeLa cell nuclear extracts active in splicing

Intervention

Purification of U1, U2, U4/U6, and U5 snRNPs via immunoaffinity chromatography using a monoclonal anti-trimethylguanosine antibody, followed by biochemical complementation assays

Outcome

Pre-mRNA splicing activity of purified snRNPssurrogate

Purified human snRNPs require additional non-snRNP protein factors to successfully catalyze pre-mRNA splicing in vitro.

Limitations

Precise quantitations of specific activities are not possible at this stage because it is unknown which snRNP is limiting or what fraction of each snRNP is in an active state.

Cite This Study

Adrian R. Krainer (1988) studied this question.

synapsesocial.com/papers/6a8581e9b451cea71dae656d https://doi.org/10.1093/nar/16.20.9415

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Pre-mRNA splicing by complementation with purified human UI, U2, U4/U6 and U5 snRNPs

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Pre-mRNA splicing by complementation with purified human UI, U2, U4/U6 and U5 snRNPs

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider