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February 1, 1988Molecular and Cellular Biology

Gel Electrophoretic Isolation of Splicing Complexes Containing U1 Small Nuclear Ribonucleoprotein Particles

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Population

in vitro splicing extracts and splicing precursor RNAs

Comparison

new system of ribonucleoprotein particle gel… vs other gel and gradient methodologies

Design

Preclinical

Authors

MZMartin ZillmannCombat Capabilities Development Command Soldier CenterMZMaria L. ZappUniversity of Massachusetts Chan Medical SchoolSBSusan M. BergetAurora St. Luke's Medical Center

Discussion

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Implication

No immediate clinical impact; leaves open validation of U1 snRNP roles in human spliceosome assembly.

Structured PICO

P
Population
in vitro splicing extracts and splicing precursor RNAs
I
Intervention
new system of ribonucleoprotein particle (RNP) gel electrophoresis
C
Comparator
other gel and gradient methodologies
O
Outcome
temporal pattern of assembly of splicing precursor RNAs into RNP complexes and retention of U1 snRNPssurrogate

A novel RNP gel electrophoresis system enables the isolation and characterization of active spliceosomes, demonstrating the essential role and retention of U1 snRNPs during complex assembly.

Cite This Study

Zillmann et al. (1988) studied this question.

synapsesocial.com/papers/6a76f3b8d8eb58afc2bd8326https://doi.org/10.1128/mcb.8.2.814-821.1988
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Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein1986 · 222 citations
  2. 2Splicing of Messenger RNA Precursors1987 · 922 citations