Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
January 1, 1996Journal of Biological ChemistryOpen Access

Internal Initiation of Translation Directed by the 5′-Untranslated Region of the mRNA for eIF4G, a Factor Involved in the Picornavirus-induced Switch from Cap-dependent to Internal Initiation

View Full Paper
Ask AI
Bookmark
Share

Population

Four different cell lines

Comparison

Insertion of the eIF4G 5'-UTR upstream of a… vs Constructs without the eIF4G 5'-UTR

Design

Preclinical

Authors

WGWeiniu GanLouisiana State UniversityRRRobert E. RhoadsUniversity of California, Riverside

Discussion

Loading...

Member takes

Implication

Supports eIF4G 5'-UTR-mediated internal initiation; leaves open its role in stress responses within cardiovascular tissues.

Structured PICO

P
Population
Four different cell lines
I
Intervention
Insertion of the eIF4G 5'-UTR upstream of a luciferase reporter gene, or between an RNA hairpin and the luciferase cistron, or between two cistrons of a bicistronic mRNA
C
Comparator
Constructs without the eIF4G 5'-UTR
O
Outcome
Translational enhancement/expression of the reporter genesurrogate

The eIF4G 5'-UTR directs internal initiation of translation, which may allow continued synthesis of eIF4G during cellular stress or viral infection when cap-dependent translation is restricted.

Cite This Study

Gan et al. (1996) studied this question.

synapsesocial.com/papers/6a85635773102ab9cdb555e4https://doi.org/10.1074/jbc.271.2.623
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation1988 · 1,377 citations
  2. 2Mapping of Functional Domains in Eukaryotic Protein Synthesis Initiation Factor 4G (eIF4G) with Picornaviral Proteases1995 · 558 citations
  3. 3Cap-independent translation by the 5' untranslated region of Theiler's murine encephalomyelitis virus1992 · 34 citations
  4. 4Internal ribosome entry site within hepatitis C virus RNA1992 · 925 citations
  5. 5Translational enhancement of the poliovirus 5' noncoding region mediated by virus-encoded polypeptide 2A.1992 · 138 citations