February 1, 1998Journal of Biological ChemistryOpen Access

Functional Characterization of the Internal Ribosome Entry Site of eIF4G mRNA

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Population

K562 cells

Design

Preclinical

Authors

WGWeiniu GanLouisiana State University MCMichael La CelleLouisiana State University RRRobert E. RhoadsUniversity of California, Riverside

Discussion

Member takes

Implication

Maps key IRES elements in eIF4G; leaves open validation in mammalian disease models.

Structured PICO

Population

K562 cells

Intervention

Bicistronic constructs with progressive deletions of the 5'-untranslated region of eIF4G mRNA and introduction of out-of-frame AUGs

Outcome

IRES-driven luciferase expression (IRES activity)surrogate

The study identifies the essential regions and mechanisms for IRES-mediated translation initiation of eIF4G mRNA, highlighting the critical role of a 3' polypyrimidine tract.

Cite This Study

Gan et al. (1998) studied this question.

synapsesocial.com/papers/6a73a3051aa2fc9e78fb6d3c https://doi.org/10.1074/jbc.273.9.5006

View Full Paper

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Bookmark

Functional Characterization of the Internal Ribosome Entry Site of eIF4G mRNA

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Functional Characterization of the Internal Ribosome Entry Site of eIF4G mRNA

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider