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October 1, 1994Journal of VirologyOpen Access

Effect of mutations downstream of the internal ribosome entry site on initiation of poliovirus protein synthesis

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Population

Poliovirus translation models (in vitro and ascites carcinoma cell extract)

Comparison

Mutations downstream of the internal ribosome… vs Unmodified/wild-type poliovirus mRNA constructs

Design

Preclinical

Authors

CHChristopher U.T. HellenBoston UniversityTPTatyana V. PestovaBoston UniversityEWEckard WimmerCalifornia Institute of Technology

Discussion

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Implication

Challenges scanning model of poliovirus initiation; leaves open whether RNA superstructure directs ribosome binding in vivo.

Structured PICO

P
Population
Poliovirus translation models (in vitro and ascites carcinoma cell extract)
I
Intervention
Mutations downstream of the internal ribosome entry site (IRES), including introduction of functional AUG codons at nt 611 or 614, removal of termination codons, and insertion of a stable hairpin
C
Comparator
Unmodified/wild-type poliovirus mRNA constructs
O
Outcome
Initiation of poliovirus translation at AUG743surrogate

The study demonstrates that not all initiation events at AUG743 in poliovirus translation can be accounted for by a scanning-dependent mechanism, suggesting a complex RNA superstructure promotes ribosome binding.

Cite This Study

Hellen et al. (1994) studied this question.

synapsesocial.com/papers/6a85635773102ab9cdb555e7https://doi.org/10.1128/jvi.68.10.6312-6322.1994
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The Initiation of Protein Synthesis Directed by the RNA from Encephalomyocarditis Virus1973 · 85 citations
  2. 2Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.1986 · 343 citations
  3. 3Translation of poliovirus RNA: role of an essential cis-acting oligopyrimidine element within the 5' nontranslated region and involvement of a cellular 57-kilodalton protein1991 · 146 citations
  4. 4Nucleotide sequences important for translation initiation of enterovirus RNA1991 · 52 citations
  5. 5Mutational analysis of upstream AUG codons of poliovirus RNA1988 · 121 citations