December 1, 1985Journal of VirologyOpen Access

Differential activation of hybrid genes containing herpes simplex virus immediate-early or delayed-early promoters after superinfection of stable DNA-transfected cell lines

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Population

DNA-transfected mouse Ltk+ cell lines and Vero cell lines harboring hybrid genes

Comparison

Superinfection with HSV type 1 or HSV type 2 vs Uninfected cells and comparison between…

Design

Preclinical

Follow-up

up to 20 h

Authors

JMJ D MoscaOsiris Therapeutics (United States)GRGregory R. ReyesCenters for Disease Control and Prevention PPPaula M. PithaRutgers, The State University of New Jersey

Discussion

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Implication

HSV infection drives high-level IFN in engineered lines; extends trans-activation models but leaves open in vivo relevance.

Structured PICO

Population

DNA-transfected mouse Ltk+ cell lines and Vero cell lines harboring hybrid genes (IE175-IFN, TK-IFN, IE175-CAT, TK-CAT)

Intervention

Superinfection with HSV type 1 or HSV type 2

Comparator

Uninfected cells (basal expression) and comparison between immediate-early (IE) and delayed-early class promoters

Outcome

Levels of gene expression (IFN-specific RNA, IFN protein product, CAT expression)surrogate

HSV superinfection specifically trans-activates hybrid genes containing immediate-early or delayed-early promoters in stable DNA-transfected cell lines.

Cite This Study

Mosca et al. (1985) studied this question.

synapsesocial.com/papers/6a8580e5f768ec88efd0c3c4 https://doi.org/10.1128/jvi.56.3.867-878.1985

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Differential activation of hybrid genes containing herpes simplex virus immediate-early or delayed-early promoters after superinfection of stable DNA-transfected cell lines

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Differential activation of hybrid genes containing herpes simplex virus immediate-early or delayed-early promoters after superinfection of stable DNA-transfected cell lines

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider