Restoration of Defective L-type Ca2+ Current in Cardiac Myocytes of Type 2 Diabetic db/db Mice by Akt and PKC-ι

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Population

Cardiac myocytes from obese type 2 diabetic db/db mice and wild-type mice

Comparison

Intracellular infusion of phosphatidylinositol… vs Wild-type cells or uninfused db/db cells

Design

Preclinical

Authors

Discussion

Hypothesis-generating in diabetic mouse models; human cardiomyocyte studies needed before any clinical consideration.

Population

Cardiac myocytes from obese type 2 diabetic db/db mice and wild-type mice

Intervention

Intracellular infusion of phosphatidylinositol 3,4,5-trisphosphate (PIP3), Akt1, Akt2, or atypical PKC-ι

Comparator

Wild-type cells or uninfused db/db cells

Outcome

L-type Ca current (ICa,L) density and voltage dependence of steady-state current activationsurrogate

A defect in PI3K/PIP3/Akt/PKC-λ signaling is mainly responsible for depressed CaV1.2 function in the hearts of type 2 diabetic db/db mice.

Lu et al. (2011) studied this question.

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