Regulation of the cross-bridge transition from a weakly to strongly bound state in skinned rabbit muscle fibers

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Population

Skinned rabbit muscle fibers

Comparison

2,3-butanedione monoxime, photogeneration of Pi… vs Absence of BDM, varying Pi concentrations, and…

Design

Preclinical

Authors

Discussion

Hypothesis-generating for cross-bridge kinetics; leaves open translation to human cardiac function.

Population

Skinned rabbit muscle fibers

Intervention

2,3-butanedione monoxime (BDM), photogeneration of Pi from caged Pi, and reduction of Ca2+ concentration

Comparator

Absence of BDM, varying Pi concentrations, and varying Ca2+ concentrations

Outcome

Rate of force development (ktr), rate of force decline (kPi), and stiffnesssurrogate

The findings support a two-step model for cross-bridge transition from a weakly bound to a force-generating state, with one step controlled by pCa.

Regnier et al. (1995) studied this question.

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