What question did this study set out to answer?

To compare the enzymatic and structural alterations of atrial and ventricular myosin in euthyroid, hypothyroid, and hyperthyroid rabbits.

February 1, 1983Circulation ResearchOpen Access

Comparative studies of atrial and ventricular myosin from normal, thyrotoxic, and thyroidectomized rabbits.

View Full Paper

Ask AI

Bookmark

Why the study?

Does thyroid hormone status alter the enzymatic and structural properties of atrial and ventricular myosin in rabbits?

Population

Euthyroid (normal), hypothyroid (thyroidectomized), and hyperthyroid (thyrotoxic) rabbits

Comparison

Hyperthyroid and hypothyroid states vs Euthyroid (normal) state

Design

Preclinical

Authors

SBS. K. BanerjeeBidhan Chandra Krishi Viswavidyalaya

Discussion

Member takes

Implication

Chamber-specific myosin regulation by thyroid status in rabbits warrants clinical caution; leaves open human atrial-ventricular translation.

Key Points

To compare the enzymatic and structural alterations of atrial and ventricular myosin in euthyroid, hypothyroid, and hyperthyroid rabbits.
Measured Ca++-, actin-activated, and K+(EDTA)-ATPase activities of atrial and ventricular myosin across euthyroid, hypothyroid, and hyperthyroid rabbit hearts.
Identified myosin isoenzyme profiles using pyrophosphate-polyacrylamide gel electrophoresis.
In euthyroid rabbits, Ca++- and actin-activated ATPase activities of atrial myosin were 2-fold greater than those of ventricular myosin and remained unchanged in hypothyroid and hyperthyroid states.
Ventricular Ca++- and actin-activated ATPase activities decreased in hypothyroid hearts (expressing solely the V3 isoenzyme) and increased in hyperthyroid hearts (expressing solely the V1 isoenzyme).
K+(EDTA)-ATPase activities for both myosins and electrophoretic mobility patterns for atrial isoenzymes (A1 and A3) remained constant across all thyroid states.

Structured PICO

Does thyroid hormone status alter the enzymatic and structural properties of atrial and ventricular myosin in rabbits?

Population

Euthyroid (normal), hypothyroid (thyroidectomized), and hyperthyroid (thyrotoxic) rabbits

Intervention

Hyperthyroid (thyrotoxic) and hypothyroid (thyroidectomized) states

Comparator

Euthyroid (normal) state

Outcome

Enzymatic (Ca++-, actin-activated, and K+ (EDTA)-ATPase activities) and structural (isoenzyme patterns via pyrophosphate-polyacrylamide gel electrophoresis) changes of ventricular and atrial myosinsurrogate

This preclinical study demonstrates that thyroid hormone regulates ventricular, but not atrial, myosin ATPase activity and isoenzyme synthesis in rabbits.

Cite This Study

S. K. Banerjee (1983) studied this question.

synapsesocial.com/papers/6a87c65a91c33485a1fe7b85 https://doi.org/10.1161/01.res.52.2.131

View Full Paper

Ask AI

Bookmark

Implication

Chamber-specific myosin regulation by thyroid status in rabbits warrants clinical caution; leaves open human atrial-ventricular translation.

Comparative studies of atrial and ventricular myosin from normal, thyrotoxic, and thyroidectomized rabbits.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Comparative studies of atrial and ventricular myosin from normal, thyrotoxic, and thyroidectomized rabbits.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider