UV irradiation modifies the poliovirus capsid, causing loss of infectivity and structural changes that are dependent on pH and the presence of reducing or ionic substances.
pH- and redox-dependent capsid changes may refine UV disinfection models; leaves open applicability to other viruses or clinical settings.
Ultraviolet (UV) irradiation of type I poliovirus resulted in a modified (M) particle that had lost infectivity, lacked ability to adsorb to HeLa cells, lacked VP4, and reduced in S value. Additional irradiation resulted in the loss of VP2, further reduction in S value, and permeability of the capsid to RNAse, This particle (C) as well as M contain the genome. Acid pH (5.5-65) and sulfhydryl-reducing substances (dithiothreitol. reduced glutathione, and L-cysteine) inhibited UV-induced modification of the capsid. UV irradiation at alkaline pH (7.5-8.5) resulted in more extensive modification of the capsid than irradiation at neutral pH. Ionic compounds were found to inhibit the modifying reaction.
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Sena et al. (1981) studied this question.
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