January 1, 1959The Journal of Immunology
Immunochemical Studies of Poliovirus
Key result
Heat or UV exposure eliminates poliovirus infectivity and alters immunologic specificity, producing denatured D-antigen.
Why the study?
Exposure of purified poliovirus to heat or ultraviolet light results in loss of infectivity and changes in immunologic specificity that require characterization.
Population
Purified poliovirus, Fraction SDGD comprising ID+ and NID+ particles
Comparison
Exposure to heat at 56°C vs exposure to ultraviolet light
Design
Immunochemical study
Authors
Discussion
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Implication
Should not change vaccine practices; leaves open D-antigen role in human immunogenicity studies.
Structured PICO
P
Population
Purified poliovirus, Fraction SDGD (comprising ID+ and NID+ particles)
I
Intervention
Exposure to heat at 56°C or ultraviolet light
O
Outcome
Loss of infectivity and change in immunologic specificity (reactivity in complement fixation tests)surrogate
Exposure of poliovirus to heat or UV light causes loss of infectivity and alters its immunologic specificity to a denatured D-antigen form.
Cite This Study
Roizman et al. (1959) studied Poliovirus. Heat at 56°C or ultraviolet light was evaluated on Loss of infectivity and change in immunologic specificity. Exposure of purified poliovirus to heat at 56°C or ultraviolet light resulted in loss of infectivity and a change in immunologic specificity, producing denatured D-antigen.