What question did this study set out to answer?

To determine how host cell factors and magnesium ion concentrations influence the thermal stability of neutralization epitopes in type 1 poliovirus.

February 1, 1985Journal of General Virology

Stabilization by Host Cell Components and Mg2+ of the Neutralization Epitopes of Poliovirus

View Full Paper

Ask AI

Bookmark

Population

Type 1 poliovirions extracted from infected HeLa cells

Comparison

Heating at 56 degrees C in the presence of 1… vs Heating with Mg2+ concentration 10 M or lower…

Design

Preclinical

Authors

BRB. RombautVrije Universiteit Brussel RVR. VrijsenVrije Universiteit Brussel ABAlbert BoeyéVrije Universiteit Brussel

Discussion

Member takes

Implication

Stabilizes poliovirus neutralization epitopes with host components and Mg2+; leaves open translation to human vaccine applications.

Key Points

To determine how host cell factors and magnesium ion concentrations influence the thermal stability of neutralization epitopes in type 1 poliovirus.
Extracted type 1 poliovirions from infected HeLa cells and heated them at 56 °C in the presence of 1 mM or ≤10^-4 M Mg2+.
Purified virions to deplete host cell components and tested epitope reconstitution by incubating purified virus with infected cell extracts at 37 °C prior to heating.
Heating poliovirus at 56 °C in 1 mM Mg2+ produced RNA-free, pluri-antigenic particles expressing neutralization epitopes, H antigen, and capsid protein VP3 epitopes.
Lowering Mg2+ concentration to 10^-4 M or below, or purifying virions to eliminate cellular components, resulted exclusively in H-antigenic particles upon heating.
Pre-incubation of purified virions with infected cell extract at 37 °C restored their capacity to form pluri-antigenic particles after heat exposure.

Structured PICO

Population

Type 1 poliovirions extracted from infected HeLa cells

Intervention

Heating at 56 degrees C in the presence of 1 mM-Mg2+ and host cell components

Comparator

Heating with Mg2+ concentration 10(-4) M or lower, or purified virus without cellular components

Outcome

Formation of RNA-free, pluri-antigenic particles possessing neutralization epitopessurrogate

Host cell components and 1 mM Mg2+ are required to stabilize the neutralization epitopes of type 1 poliovirus during heating at 56 degrees C.

Cite This Study

Rombaut et al. (1985) studied this question.

synapsesocial.com/papers/6a835c1c9ef178b4bb8cf2de https://doi.org/10.1099/0022-1317-66-2-303

View Full Paper

Ask AI

Bookmark

Stabilization by Host Cell Components and Mg2+ of the Neutralization Epitopes of Poliovirus

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Stabilization by Host Cell Components and Mg2+ of the Neutralization Epitopes of Poliovirus

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider