April 30, 2009Journal of VirologyOpen Access

Theiler's Murine Encephalomyelitis Virus Leader Protein Is the Only Nonstructural Protein Tested That Induces Apoptosis When Transfected into Mammalian Cells

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Population

Baby hamster kidney (BHK-21) cells and M1-D macrophages

Comparison

Transfection to express individual nonstructural… vs Expression of other nonstructural proteins, or…

Design

Preclinical

Authors

JFJi-Lao FanKyushu University KSKyung‐No SonUniversity of Michigan SASevim Yildiz ArslanThe Graduate Center, CUNY

Discussion

Member takes

Implication

L-protein CHCC motif drives apoptosis in TMEV cell models; leaves open its role in viral persistence and requires in vivo confirmation.

Structured PICO

Population

Baby hamster kidney (BHK-21) cells and M1-D macrophages

Intervention

Transfection to express individual nonstructural genes (except 3B) of the low-neurovirulence BeAn virus strain, specifically the leader (L) protein

Comparator

Expression of other nonstructural proteins, or mutated L protein (lacking the CHCC Zn finger motif)

Outcome

Apoptosis (assessed by FACS of propidium iodide- and annexin V-stained cells, immunoblot analysis of PARP and caspase cleavages, and electron microscopy)surrogate

The TMEV leader protein is the sole nonstructural protein tested that induces apoptosis in mammalian cells, a process dependent on its CHCC Zn finger motif.

Cite This Study

Fan et al. (2009) studied this question.

synapsesocial.com/papers/6a87d35ff762332bf8fe8264 https://doi.org/10.1128/jvi.00353-09

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Theiler's Murine Encephalomyelitis Virus Leader Protein Is the Only Nonstructural Protein Tested That Induces Apoptosis When Transfected into Mammalian Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Theiler's Murine Encephalomyelitis Virus Leader Protein Is the Only Nonstructural Protein Tested That Induces Apoptosis When Transfected into Mammalian Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider