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October 1, 2000RNAOpen Access

Interaction of the eIF4G initiation factor with the aphthovirus IRES is essential for internal translation initiation in vivo

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Population

FMDV-susceptible cell extracts and transfected living cells

Comparison

Substitutions in the conserved internal AA loop… vs Wild-type IRES or substitutions in the vicinity…

Design

Preclinical

Authors

SQSonia López de QuintoEuropean Molecular Biology LaboratoryEMEncarnación Martı́nez-SalasConsejo Superior de Investigaciones Científicas

Discussion

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Implication

May inform antiviral strategies targeting FMDV IRES; leaves open therapeutic translation beyond this animal model.

Structured PICO

P
Population
FMDV-susceptible cell extracts and transfected living cells
I
Intervention
Substitutions in the conserved internal AA loop of the base of domain 4 of the foot-and-mouth disease virus (FMDV) IRES
C
Comparator
Wild-type IRES or substitutions in the vicinity of the internal AA loop
O
Outcome
eIF4G-RNA interaction in vitro and IRES-dependent translation initiation in vivosurrogate

The interaction between the eIF4G initiation factor and domain 4 of the FMDV IRES is essential for internal translation initiation, suggesting eIF4G acts as a linker to recruit translational machinery.

Cite This Study

Quinto et al. (2000) studied this question.

synapsesocial.com/papers/6a87e81d350e059174ad3f7ahttps://doi.org/10.1017/s1355838200000753
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Functional Dissection of Eukaryotic Initiation Factor 4F: the 4A Subunit and the Central Domain of the 4G Subunit Are Sufficient To Mediate Internal Entry of 43S Preinitiation Complexes1996 · 350 citations
  2. 2Canonical Eukaryotic Initiation Factors Determine Initiation of Translation by Internal Ribosomal Entry1996 · 500 citations
  3. 3eIF4 Initiation Factors: Effectors of mRNA Recruitment to Ribosomes and Regulators of Translation1999 · 2,136 citations
  4. 4A prokaryotic-like mode of cytoplasmic eukaryotic ribosome binding to the initiation codon during internal translation initation of hepatitis C and classical swine fever virus RNAs1998 · 735 citations
  5. 5eIF4G--A Multipurpose Ribosome Adapter?1997 · 226 citations