September 1, 1999Journal of General Virology

Functional features of the bovine enterovirus 5′-non-translated region

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Population

Full-length coxsackievirus B3 cDNA plasmids and SP6 transcripts containing 5'-NTRs fused to the luciferase…

Design

Preclinical

Authors

RZRoland ZellJena University Hospital KSKarim SidigiFriedrich Schiller University Jena AHAndreas HenkeJena University Hospital

Discussion

Member takes

Implication

Does not inform clinical practice; leaves open species-specific requirements for enteroviral cloverleaf and IRES function.

Structured PICO

Population

Full-length coxsackievirus B3 (CVB3) cDNA plasmids and SP6 transcripts containing 5'-NTRs fused to the luciferase message

Intervention

Engineering hybrid 5'-NTRs by exchanging the CVB3 cloverleaf with the BEV1 genome region, and deleting specific domains (domain I**, first or second BEV cloverleaf)

Outcome

Viral viability and infectivitysurrogate

The study demonstrates that the two BEV cloverleaf structures can functionally replace the CVB3 cloverleaf to yield viable virus, but BEV IRES sequences cannot replace CVB3 IRES sequences.

Cite This Study

Zell et al. (1999) studied this question.

synapsesocial.com/papers/6a87f19ed79ae02bd34de6e3 https://doi.org/10.1099/0022-1317-80-9-2299

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Functional features of the bovine enterovirus 5′-non-translated region

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Functional features of the bovine enterovirus 5′-non-translated region

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider