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August 11, 2017Developmental Pharmacology and Therapeutics

Developmental Changes in Alpha Adrenergic Effects on Canine Purkinje Fiber Automaticity

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Why the study?

Does phenylephrine alter the automatic discharge rate differently in neonatal versus adult canine cardiac Purkinje fibers?

Population

Isolated neonatal (less than 10 days, n=23) and adult (n=32) canine cardiac Purkinje fibers

Comparison

Superfusion with 5 X 10 M phenylephrine… vs Comparison between neonatal and adult fibers…

Design

Preclinical

Key result

Phenylephrine decreased automatic rate in 75% of adult and 52% of neonatal canine Purkinje fibers, while causing an alpha-adrenergic-induced acceleration in the remaining neonates not seen in adults.

Authors

RRRobert F. RederPDPeter DaniloMRMichael R. Rosen

Discussion

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Overview

Suggests age-dependent differences in alpha-adrenergic receptor function; leaves open whether these developmental.

Structured PICO

Does phenylephrine alter the automatic discharge rate differently in neonatal versus adult canine cardiac Purkinje fibers?

P
Population
55 isolated neonatal (<10 days) and adult canine cardiac Purkinje fibers studied for automaticity responses to phenylephrine.
I
Intervention
Superfusion with 5 X 10(-8) M phenylephrine dissolved in Tyrode's solution
C
Comparator
Comparison between neonatal and adult fibers, and response to blocking agents (phentolamine, propranolol)
O
Outcome
Change in automatic discharge ratesurrogate

Main Result

Absolute Event Rate: 75% vs 52%

Neonatal canine Purkinje fibers exhibit an alpha-adrenergic-induced acceleration of automatic rate that is not seen in adults, suggesting developmental changes in autonomic receptor function.

Cite This Study

Reder et al. (2017) studied Canine cardiac Purkinje fiber automaticity (n=55). Phenylephrine vs. Adult vs Neonatal fibers was evaluated on Decrease in rate of automatic discharge. Phenylephrine decreased automatic rate in 75% of adult and 52% of neonatal canine Purkinje fibers, while causing an alpha-adrenergic-induced acceleration in the remaining neonates not seen in adults.

synapsesocial.com/papers/6a8820678c7e900eb714c063https://doi.org/10.1159/000457150
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Effects of adrenergic amines on electrophysiological properties and automaticity of neonatal and adult canine Purkinje fibers: evidence for alpha- and beta-adrenergic actions.1977 · 126 citations
  2. 2Specific alpha 1-adrenergic receptor subtypes modulate catecholamine-induced increases and decreases in ventricular automaticity.1990 · 81 citations
  3. 3Positive chronotropic responses induced by alpha 1-adrenergic stimulation of normal and "ischemic" Purkinje fibers have different receptor-effector coupling mechanisms.1992 · 22 citations
  4. 4Neuronal regulation of the development of the alpha-adrenergic chronotropic response in the rat heart.1985 · 65 citations
  5. 5Sympathetic Neural and α‐Adrenergic Modulation of Arrhythmias<sup>a</sup>1988 · 17 citations