January 25, 2011Journal of Biological ChemistryOpen Access

Localization of the Dantrolene-binding Sequence near the FK506-binding Protein-binding Site in the Three-dimensional Structure of the Ryanodine Receptor

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Population

Skeletal and cardiac ryanodine receptors, specifically GFP-RyR2 fusion proteins

Design

Preclinical

Authors

RWRuiwu WangElectrophysiology XZXiaowei ZhongLongyan University XMXing MengShandong University of Technology

Discussion

Member takes

Implication

Animal RyR mapping leaves open dantrolene's clinical mechanism; hypothesis-generating for allosteric stabilization pending human validation.

Structured PICO

Population

Skeletal (RyR1) and cardiac (RyR2) ryanodine receptors, specifically GFP-RyR2 fusion proteins (RyR2Arg-626-GFP and RyR2Tyr-846-GFP)

Intervention

GFP insertion and FRET pair generation for three-dimensional cryo-EM mapping

Outcome

Three-dimensional location of the dantrolene-binding sequencesurrogate

The study maps the dantrolene-binding sequence to domain 9 near the FKBP12.6-binding site on the ryanodine receptor, providing structural insight into its allosteric stabilizing mechanism.

Cite This Study

Wang et al. (2011) studied this question.

synapsesocial.com/papers/6a8906f36fb350aba9126a18 https://doi.org/10.1074/jbc.m110.194316

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Implication

Animal RyR mapping leaves open dantrolene's clinical mechanism; hypothesis-generating for allosteric stabilization pending human validation.

Localization of the Dantrolene-binding Sequence near the FK506-binding Protein-binding Site in the Three-dimensional Structure of the Ryanodine Receptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Localization of the Dantrolene-binding Sequence near the FK506-binding Protein-binding Site in the Three-dimensional Structure of the Ryanodine Receptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider