September 28, 1999Circulation
1166 A/C Polymorphism of the Angiotensin II Type 1 Receptor Gene and the Response to Short-Term Infusion of Angiotensin II
Why the study?
Does the 1166 A/C polymorphism of the Angiotensin II type 1 receptor gene affect the blood-pressure, renal hemodynamic, and aldosterone response to infused Angiotensin II in young, male, white subjects?
Population
116 young, male, white volunteers with normal or mildly elevated blood pressure on a high salt intake.
Comparison
Two doses of Angiotensin II infusion. vs Comparison between genotypes: subjects…
Design
Cohort
Follow-up
60 minutes (two 30-minute infusions)
Authors
Discussion
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Implication
No support for genotyping this polymorphism to guide Ang II-related care in young white men; leaves open relevance in other groups or conditions.
Key Points
- To evaluate whether the 1166 A/C polymorphism of the angiotensin II type 1 receptor gene influences blood pressure, renal hemodynamic, or aldosterone responses to short-term angiotensin II infusion.
- Genotyped 116 young, white male volunteers (65 normotensive, 51 mildly hypertensive) maintained on a high salt intake for the 1166 A/C polymorphism.
- Administered two consecutive 30-minute systemic infusions of angiotensin II (0.5 and 3 ng·kg⁻¹·min⁻¹) to measure changes in blood pressure, plasma aldosterone, glomerular filtration rate, filtration fraction, and renal blood flow.
- Angiotensin II infusion increased blood pressure, plasma aldosterone, glomerular filtration rate, and filtration fraction, while decreasing renal blood flow across all participants.
- Blood pressure, aldosterone, and renal hemodynamic changes did not differ significantly between AA homozygotes (n=56), AC heterozygotes (n=47), and CC homozygotes (n=13).
- No significant differences in vascular or hormonal responsiveness appeared when comparing AA homozygotes against pooled C-allele carriers (n=60) or when restricting analyses to normotensive subjects.
Structured PICO
Does the 1166 A/C polymorphism of the Angiotensin II type 1 receptor gene affect the blood-pressure, renal hemodynamic, and aldosterone response to infused Angiotensin II in young, male, white subjects?
P
Population
116 young, male, white volunteers with normal (n = 65) or mildly elevated (n = 51) blood pressure on a high salt intake.
I
Intervention
Two doses of Angiotensin II infusion (0.5 and 3 ng/kg/min over 30 minutes each).
C
Comparator
Comparison between genotypes: subjects homozygous for the A allele (n = 56) vs heterozygous subjects (n = 47) vs subjects homozygous for the C allele (n = 13), and A allele homozygotes vs all C allele carriers pooled (n = 60).
O
Outcome
Blood pressure, plasma aldosterone, glomerular filtration rate, filtration fraction, and renal blood flow response to infused Angiotensin II.surrogate
The 1166 A/C polymorphism of the Angiotensin II type 1 receptor gene does not significantly alter blood pressure, aldosterone, or renal vascular responses to infused Angiotensin II in young, white men.
Cite This Study
Hilgers et al. (1999) studied this question.