What question did this study set out to answer?

To identify and structurally characterize the atrial natriuretic factor receptor in bovine adrenal zona glomerulosa membranes.

February 1, 1986Journal of Biological ChemistryOpen Access

Affinity cross-linking of atrial natriuretic factor to its receptor in bovine adrenal zona glomerulosa.

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Population

Bovine adrenal zona glomerulosa membranes

Comparison

125I-Labeled atrial natriuretic factor… vs Unlabeled ANF, angiotensin II…

Design

Preclinical

Authors

SMSylvain MelocheInstitute for Research in Immunology and Cancer HOHuy OngPerdana University MCM. CantinHeart Failure / Cardiomyopathy

Discussion

Member takes

Implication

Candidate ANF receptor proteins identified in bovine adrenal; leaves open functional validation and human relevance.

Key Points

To identify and structurally characterize the atrial natriuretic factor receptor in bovine adrenal zona glomerulosa membranes.
Cross-linked iodine-125-labeled atrial natriuretic factor to bovine adrenal zona glomerulosa membranes using the hydrophilic agent bis(sulfosuccinimidyl) suberate.
Resolved labeled membrane proteins via sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing and non-reducing conditions, in the presence and absence of protease inhibitors and competing ligands.
Identified two specifically labeled receptor bands with apparent molecular weights of 68,000 and 114,000 Da.
Demonstrated dose-dependent inhibition of cross-linking by unlabeled atrial natriuretic factor beginning at 10⁻¹⁰ M, with no competition from angiotensin II or adrenocorticotropic hormone.
Confirmed that the two labeled species are not products of artifactual proteolysis or cleavage from larger disulfide-linked complexes.

Structured PICO

Population

Bovine adrenal zona glomerulosa membranes

Intervention

125I-Labeled atrial natriuretic factor (ANF) cross-linking using bis(sulfosuccinimidyl) suberate

Comparator

Unlabeled ANF, angiotensin II, adrenocorticotropic hormone, and liver membranes

Outcome

Identification and molecular weight characterization of ANF binding sites/receptorssurrogate

The study identifies two specific protein components (Mr 68,000 and 114,000) as putative ANF receptors in bovine adrenal zona glomerulosa.

Cite This Study

Meloche et al. (1986) studied this question.

synapsesocial.com/papers/6a8a3c64a90c2f4c44fbb296 https://doi.org/10.1016/s0021-9258(17)35971-9

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Affinity cross-linking of atrial natriuretic factor to its receptor in bovine adrenal zona glomerulosa.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Affinity cross-linking of atrial natriuretic factor to its receptor in bovine adrenal zona glomerulosa.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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