Key result
Incubation of bovine adrenal zona glomerulosa membranes with 125I-ANF followed by u.v. irradiation resulted in irreversible labelling of the ANF-R1 receptor, requiring aerobic conditions and lipids.
Underivatized 125I-ANF can be used for photoaffinity labelling of the ANF-R1 receptor, providing a simple method to characterize its hormone-binding domain.
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May aid in vitro ANF-R1 domain mapping in animal models; leaves open any clinical relevance or human translation.
Larose et al. (1990) studied this question. 125I-ANF-(99-126) and u.v. irradiation was evaluated on Irreversible labelling of the ANF-RI receptor. Incubation of bovine adrenal zona glomerulosa membranes with 125I-ANF followed by u.v. irradiation resulted in irreversible labelling of the ANF-R1 receptor, requiring aerobic conditions and lipids.
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