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July 1, 1985Journal of Biological ChemistryOpen Access

Photoaffinity labeling of atrial natriuretic factor receptors of rat kidney cortex plasma membranes.

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Population

Purified plasma membranes from rat kidney cortex, liver, and adipocytes

Comparison

Incubation with a radioactive photoaffinity probe vs Incubation with reduced and alkylated ANF or…

Design

Preclinical

Authors

CYChristopher YipUniversity of TorontoLLLeslie LaingQueen's UniversityTFT.G. FlynnQueen's University

Discussion

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Implication

No change to clinical practice indicated; leaves open the 140-kDa glycoprotein as ANF receptor candidate for validation.

Structured PICO

P
Population
Purified plasma membranes from rat kidney cortex, liver, and adipocytes
I
Intervention
Incubation with a radioactive photoaffinity probe (synthetic rat atrial natriuretic factor derivatized with the N-hydroxysuccinimide ester of [125I]iodoazidosalicylic acid)
C
Comparator
Incubation with reduced and alkylated ANF or other unrelated peptides
O
Outcome
Identification and specific labeling of the ANF receptor proteinsurrogate

This preclinical study identifies a 140-kDa glycoprotein as the likely atrial natriuretic factor (ANF) receptor in rat kidney and liver plasma membranes.

Cite This Study

Yip et al. (1985) studied this question.

synapsesocial.com/papers/6a7ea09f6cdd7a4db3026513https://doi.org/10.1016/s0021-9258(17)39458-9
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Specific membrane receptors for atrial natriuretic factor in renal and vascular tissues.1984 · 262 citations
  2. 2Atrial natriuretic factor selectively activates particulate guanylate cyclase and elevates cyclic GMP in rat tissues.1984 · 675 citations
  3. 3The Structure of Rat Preproatrial Natriuretic Factor as Defined by a Complementary DNA Clone1984 · 232 citations
  4. 4Alignment of Rat Cardionatrin Sequences with the Preprocardionatrin Sequence from Complementary DNA1985 · 120 citations
  5. 5Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane1971 · 8,565 citations